Journal: Cancer Reports

Article Title: Radiation‐induced effects on TGF‐β and PDGF receptor signaling in cancer‐associated fibroblasts

doi: 10.1002/cnr2.2018

Figure Lengend Snippet: List of secondary antibodies used for western blot and immunocytochemistry.

Article Snippet: Donkey anti Sheep/Goat IgG:HRP , Serotec , STAR88P , 1:10000.

Techniques: Western Blot, Immunocytochemistry

Journal: The Journal of Cell Biology

Article Title: Neurite Fasciculation Mediated by Complexes of Axonin-1 and Ng Cell Adhesion Molecule

doi:

Figure Lengend Snippet: Analysis of the molecular masses of wild-type NgCAM and NgCAM domain deletion mutants transiently expressed in COS7 cells. Total protein of nontransfected COS cells ( COS ) and COS cells transfected with wild-type NgCAM ( wild-type ) and the and NgCAM single ( A ) and double ( B ) domain deletion mutants was separated by SDS-PAGE, transferred to nitrocellulose and immunostained using a polyclonal anti-NgCAM antibody followed by enhanced chemiluminescence detection. Molecular masses are indicated.

Article Snippet: Cy3-conjugated anti– mouse IgG and Texas red–conjugated anti–rabbit IgG was from Zymed Laboratories Inc. (South San Francisco, CA), FITC-conjugated anti–goat IgG was from Jackson ImmunoResearch Laboratories (West Grove, PA), and anti–sheep/goat IgG secondary antibodies for enhanced chemiluminescence (ECL) detection were from Boehringer Mannheim (Mannheim, Germany).

Techniques: Transfection, SDS Page

Journal: The Journal of Cell Biology

Article Title: Neurite Fasciculation Mediated by Complexes of Axonin-1 and Ng Cell Adhesion Molecule

doi:

Figure Lengend Snippet: Axonin-1 binding to wild-type NgCAM and the domain deletion mutants ΔIg56, ΔIg6Fn1, ΔFn12, ΔIg56 Fn1, ΔIg6Fn12, and ΔIg56Fn12. ( A ) Analysis of the molecular masses of the NgCAM domain deletion mutants ΔIg56Fn1, ΔIg6Fn12, and ΔIg56Fn12 transiently expressed in COS7 cells. Western blot analysis was performed as described in Fig. , using a polyclonal anti-NgCAM antibody followed by enhanced chemiluminescence for detection. Molecular masses are indicated. ( B ) Binding of axonin-conjugated TRITC Covaspheres to COS cells expressing wild-type NgCAM and the domain deletion mutants ΔIg56, ΔIg6Fn1, ΔFn12, ΔIg56Fn1, ΔIg6Fn12, and ΔIg56Fn12. The detection of NgCAM expressing cells and the quantification was carried out as described in Fig. . Each column corresponds to three independent experiments (± SD).

Article Snippet: Cy3-conjugated anti– mouse IgG and Texas red–conjugated anti–rabbit IgG was from Zymed Laboratories Inc. (South San Francisco, CA), FITC-conjugated anti–goat IgG was from Jackson ImmunoResearch Laboratories (West Grove, PA), and anti–sheep/goat IgG secondary antibodies for enhanced chemiluminescence (ECL) detection were from Boehringer Mannheim (Mannheim, Germany).

Techniques: Binding Assay, Western Blot, Expressing

Journal: The Journal of Cell Biology

Article Title: Neurite Fasciculation Mediated by Complexes of Axonin-1 and Ng Cell Adhesion Molecule

doi:

Figure Lengend Snippet: Binding of axonin-1 to the NgCAM mutants NgCAM PM1-3, NgCAM ΔP871-V888, and wild-type NgCAM. ( A ) Schematic representation of the point mutations introduced into positions R865, R864, and R862 of NgCAM PM1, NgCAM PM2, and NgCAM PM3. Amino acid exchanges are underlined and the site of cleavage marked by an arrow. The relative positions of the residues with respect to the cleavage site are indicated. ( B ) Western blot analysis. The NgCAM mutants were transiently expressed in COS7 cells, total protein separated by SDS-PAGE, transferred to nitrocellulose and immunostained using a monoclonal anti-NgCAM antibody against the 135-kD fragment followed by enhanced chemiluminescence for detection. Molecular masses are indicated. ( C ) Binding of axonin-conjugated TRITC Covaspheres to COS cells expressing wild-type NgCAM and the NgCAM mutants NgCAM PM1-3, NgCAM ΔP871-V888. The identification of NgCAM expressing cells and the quantification of the binding of axonin-1 Covaspheres was carried out as described in Fig. . Each column corresponds to three independent experiments (± SD).

Article Snippet: Cy3-conjugated anti– mouse IgG and Texas red–conjugated anti–rabbit IgG was from Zymed Laboratories Inc. (South San Francisco, CA), FITC-conjugated anti–goat IgG was from Jackson ImmunoResearch Laboratories (West Grove, PA), and anti–sheep/goat IgG secondary antibodies for enhanced chemiluminescence (ECL) detection were from Boehringer Mannheim (Mannheim, Germany).

Techniques: Binding Assay, Western Blot, SDS Page, Expressing